magnetic cell separation kits (Miltenyi Biotec)

Miltenyi Biotec is a verified supplier

Miltenyi Biotec manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Miltenyi Biotec magnetic cell separation kits
Magnetic Cell Separation Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+separation+kit/Myeloid+Dendritic+Cell+Isolation+Kit%2C+human/pm42520804-271-13-17
Average 94 stars, based on 37 article reviews

magnetic cell separation kits - by Bioz Stars, 2026-10

94/100 stars

Images

Isolation:

Article Title: Substituted indazoles useful for treatment and prevention of allergic and/or inflammatory diseases in animals
Article Snippet: To detect the effect of the present compounds on IL-23 production, human primary monocytes (isolated from human PBMCs using magnetic separation [Miltenyi Biotech, Monocyte Isolation Kit, Cat. No. 130-091-153] and by the addition of growth factors (recombinant human GM-CSF [PeproTech, Cat. No. 300-03] and IL-4 [PeproTech, Cat. No. 200-04]) in complete medium (VLE (very low endotoxin) RPMI 1640 [Biochrom AG, Cat. No. FG1415], 10% Fetal Bovine Serum (FBS) [Gibco, Cat-No. 10493-106]; 50 μM β-mercaptoethanol (Gibco, Cat. No. 31350], 50 U/ml penicillin and streptomycin [Gibco, Cat. No. 15140-114]) were differentiated in culture over 6 days to DCs. .. For this purpose, human PBMCs were isolated from whole blood as described above and the plasmacytoid DCs (pDCs) were isolated therefrom using a commercially available cell separation kit (Miltenyi Biotech, Plasmacytoid Dendritic Cell Isolation Kit II, Cat. No. 130-097-415). .. The obtained pDCs were resuspended in complete medium (RPMI 1640+GlutaMax [Gibco, Cat. No. 61870-010] supplemented with 10% FBS [Gibco, Cat. No. 10493-106] and 50 U penicillin/streptomycin [Gibco, Cat. No. 15140-114]) and seeded at a cell density of 5×104 cells/well in a 96-well microtitre plate (Costar, Cat. No. 3599).

Article Title: Substituted indazoles, methods for the production thereof, pharmaceutical preparations that contain said new substituted indazoles, and use of said new substituted indazoles to produce drugs
Article Snippet: .. For this purpose, human PBMCs were isolated from whole blood as described above and the plasmacytoid DCs (pDCs) were isolated therefrom using a commercially available cell separation kit (Miltenyi Biotech, Plasmacytoid Dendritic Cell Isolation Kit II, Cat. No. 130-097-415). .. Thereafter, the DCs were stimulated to produce IL-23 by the addition of 10 ng/ml LPS (Sigma, Escherichia coli serotype 0127: B8, Cat. No. L3129) (TLR4 ligand) and 2.5 μg/ml of TLR-7/8 ligand R848 (Invivogen, Cat. No. tlrl-r848-5), both activate the IRAK4-mediated signalling pathway, in an incubator (37° C., 95% rH, 5% CO2) for 24 hours.

Article Title: SNA·SMNP·CBE system: A novel integrative strategy for β-hemoglobinopathies gene therapy
Article Snippet: .. Method 2: Immno-magnetic isolation (IMS) For CD34 microbead magnetic-activated cell sorting isolation, a cell separation kit (CD34 MicroBead Kit, Miltenyi Biotec, 130–046–702) was used, as per the guided instruction. ..

Cell Isolation:

Article Title: Fibroblast-specific MyD88-dependent signaling aggravates inflammation and cardiac dysfunction in the MI heart
Article Snippet: .. To enrich the FB population, endothelial (CD31+) and myeloid (CD45+) cells were sorted using the Magnetic Cell Isolation and Cell Separation kit (Miltenyi Biotec) with recommended dilutions of antibodies against CD31 (Miltenyi Biotec, #130-097-418) and CD45 (Miltenyi Biotec, #130-052-301) as per the manufacturer’s instructions. .. Primary cultures of neonatal rat ventricular fibroblasts (NRVFs) were prepared from 1–2-day-old Sprague-Dawley rats as described previously.

Article Title: Fibroblast-specific MyD88-dependent signaling aggravates inflammation and cardiac dysfunction in the MI heart.
Article Snippet: Jo ur na l P re -p ro of Journal Pre-proof Background: Excessive fibrosis and chronic inflammation are vital to adverse cardiac remodeling of an MI heart.. The crosstalk of fibroblasts (FBs) (primary drivers of fibrosis) and immune cells (that govern inflammation) is critical for the repair and remodeling of the injured heart.. However, the molecular mechanisms through which FBs communicate with immune cells are poorly understood.

Article Title: Fibroblast-Specific Depletion of Human Antigen R Alleviates Myocardial Fibrosis Induced by Cardiac Stress
Article Snippet: .. In some experiments, fibroblasts were enriched by using the Magnetic cell isolation and cell separation kit (catalog no. 130-042-301; Miltenyi Biotec) with recommended dilutions of antibodies against CD31 (catalog no. 130-097-418; Miltenyi Biotec) and CD45 (catalog no. 130-052-301; Miltenyi Biotec) as per the manufacturer’s instructions. .. Human ventricular cardiac fibroblasts (designated as HCFs; catalog no. CC-2904; Lonza) were cultured in FGM-3 Cardiac Fibroblast Growth Medium-3 BulletKit (catalog no. CC-4526; Lonza) in a humified incubator at 37°C with 5% carbon dioxide.

Article Title: Cardiac Fibroblast GSK-3α Aggravates Ischemic Cardiac Injury by Promoting Fibrosis, Inflammation, and Impairing Angiogenesis
Article Snippet: .. To enrich the FB population, endothelial (CD31+) and myeloid (CD45+) cells were sorted using the Magnetic Cell Isolation and Cell Separation kit (Miltenyi Biotec) with recommended dilutions of antibodies - CD31 (Miltenyi Biotec, #130-097-418) and CD45 (Miltenyi Biotec, #130-052-301) as per the manufacturer’s instructions. ..